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reagent kits  (Bio-Rad)


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    Bio-Rad reagent kits
    Reagent Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 26016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reagent kits/product/Bio-Rad
    Average 96 stars, based on 26016 article reviews
    reagent kits - by Bioz Stars, 2026-05
    96/100 stars

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    a ) CD4 or CD8 T cells were enriched using AutoMACS selection, lysed and OPA1 presence determined. Shown is a representative Western blot from three biological repeats. b-e ) Memory CD8 T cells were isolated from spleens from YME1L1 f/f littermate controls and YME1L1 ΔTB animals, purified via flow sorting (CD8 + CD44 hi ), and analysed using an electron microscope. b) Representative EM images, were processed to segment mitochondria and cristae respectively, and measurements were made. c) Mitochondrial section area (nm2), d) Mitochondrial section roundness (4π·Area/Perimeter² in arbitrary units (a.u.)) (n=94 controls, n=37 YME1L1 ΔTB ), e) Percentage of branched cristae (n=29 controls, n=9 YME1L1 ΔTB ), f) Cristae Feret diameter (nm), and g) Tortuosity calculated as 1-Feret diameter (FD)/longest shortest path (LSP) (n=371 controls, n=138 YME1L1 ΔTB ). Statistical analysis: Mann-Whitney tests.

    Journal: bioRxiv

    Article Title: YME1L1 is Dispensable for T Lymphocyte Activation Despite its Upregulation and Activity

    doi: 10.64898/2026.03.16.712079

    Figure Lengend Snippet: a ) CD4 or CD8 T cells were enriched using AutoMACS selection, lysed and OPA1 presence determined. Shown is a representative Western blot from three biological repeats. b-e ) Memory CD8 T cells were isolated from spleens from YME1L1 f/f littermate controls and YME1L1 ΔTB animals, purified via flow sorting (CD8 + CD44 hi ), and analysed using an electron microscope. b) Representative EM images, were processed to segment mitochondria and cristae respectively, and measurements were made. c) Mitochondrial section area (nm2), d) Mitochondrial section roundness (4π·Area/Perimeter² in arbitrary units (a.u.)) (n=94 controls, n=37 YME1L1 ΔTB ), e) Percentage of branched cristae (n=29 controls, n=9 YME1L1 ΔTB ), f) Cristae Feret diameter (nm), and g) Tortuosity calculated as 1-Feret diameter (FD)/longest shortest path (LSP) (n=371 controls, n=138 YME1L1 ΔTB ). Statistical analysis: Mann-Whitney tests.

    Article Snippet: Cell suspensions were incubated with CD4-, CD8- or CD19-APC antibodies (Supplementary Table 1) and subsequently with anti-APC Microbeads (Milteny Biotech), for the isolation of CD4+ or CD8+ T cells or CD19+ B cells, respectively, by the autoMACS Pro Separator (Milteny Biotech).

    Techniques: Selection, Western Blot, Isolation, Purification, Microscopy, MANN-WHITNEY